Are you absolutely sure your peptides are stable in your storage or biological assay conditions ?

One of the most important aspects of high quality research is the reproducibility of obtained results. The key of the reproducibility is the stability of the product during the storage, manipulation of compounds and influence of the assay conditions.

 

Stored samples

 

What could go wrong ?

Peptides are often very active compounds composed of different, natural or non-natural amino acids. Certain of them can be sensitive to different environmental conditions like temperature, pH, light, oxygen or additives. During prolonged exposure times or during daily manipulations (opening/closing of vials, freezing/defreezing of samples) they can undergo multiple degradation pathways. The most common are oxidation, deamination and hydrolysis. Additionally, introduction of other motifs, like cyclizations, phosphorylations or glycolsylations can even more complicate the process. To prevent this, it is important to know how peptides behave during biological assays or short and long term storage conditions.

 

graphic concentration and activity of a peptide

 

How we can help:

GENEPEP provides customized stability studies on your peptides. Equipped with high-precision Memmert stability chambers, we propose different solutions starting from simple short term storage conditions to ICH compliant long term programs.

 

Researchers in a lab

Storage stability tests

Simple constant temperature in time storage conditions:

  • Temperature range: -20°C to +70°C
  • Time range examples: 3 months, 6 months, 12 months

ICH Q1A compliant storage studies:

  • Long term studies: 25°C and 60% RH for 12 months, or 30°C and 65% RH for 12 months
  • Intermediate studies: 30°C and 65% RH for 6 months
  • Accelerated studies: 40°C and 75% RH for 6 months

 

Stress stability tests

Storage in specific biological assay composition:
different buffers, additives (DMSO, solvents, etc)

Exposure to:

  • light
  • different pH
  • high temperatures

 

Impact of daily manipulation tests

  • opening/closing of vials, freezing/defreezing of samples
  • influence of storage form – lyophilized, solution

 

Follow up

Based on the results of the stability studies, our experts in peptide chemistry can help in design and synthesis of new analogs of your peptides to improve its stability.